ABSTRACT
Abstract There is growing evidence that C. albicans is associated with dental caries, but its role on caries development needs to be better clarified. Objective: To evaluate at the hard tissue level the effect of C. albicans on the cariogenic potential of S. mutans biofilms focusing on the mineral profile of induced carious lesions. This study also aimed to evaluate the effect of C. albicans on the acidogenic potential of S. mutans biofilms. Methodology: Dual-species (CA+SM) and single-species biofilms (CA or SM) were grown on the surface of enamel slabs in the presence of glucose/sucrose supplemented culture medium for 24, 48 and 72 hours. Demineralization was evaluated through percentage of surface microhardness change (%SMC) and transversal microradiography analysis (ILM and LD) and pH of the spent medium was recorded daily. Data were analyzed by two-way ANOVA followed by Bonferroni correction. Results: %SMC was statistically different among the biofilms at each time point being the highest for SM biofilms and the lowest for CA biofilms which also differed from CA+SM biofilms [SM (24 h: 47.0±7.3; 48 h: 66.3±8.3; 72 h: 75.4±3.9); CA (24 h: 7.3±3.3; 48 h: 7.1±6.4; 72 h: 6.6±3.6); CA+SM (24 h: 35.9±7.39.1; 48 h: 47.2±9.5; 72 h: 47.6±9.5)]. pH of spent medium was statistically lower for SM biofilms compared to the other biofilms at each time point and remained constant over time while pH values increased from 24 to 72 h for both CA and CA+SM biofilms [SM (24 h: 4.4±0.1; 48 h: 4.4±0.1; 72 h: 4.5±0.1); CA (24 h: 6.9±0.3; 48 h: 7.2±0.2; 72 h: 7.5±0.2); CA+MS (24 h: 4.7±0.2; 48 h: 5.1±0.1; 72 h: 6.1±0.6)]. IML and LD for SM biofilms increased over time while no difference was observed from 24 to 72 h for the other biofilms. Conclusions: The present data suggest that C. albicans has low enamel demineralization potential and the presence of C. albicans can reduce both the cariogenic and acidogenic potentials of S. mutans biofilms.
Subject(s)
Animals , Cattle , Streptococcus mutans/metabolism , Candida albicans/physiology , Tooth Demineralization/microbiology , Biofilms/growth & development , Dental Enamel/microbiology , Reference Values , Surface Properties , Time Factors , Acids/metabolism , Microradiography/methods , Colony Count, Microbial , Dental Enamel/chemistry , Hardness Tests , Hydrogen-Ion ConcentrationABSTRACT
Objetivo: relatar o diagnóstico e tratamento de um possível papiloma escamoso em uma criança por meio da criocirurgia com nitrogênio líquido. Relato de caso: Paciente de 8 anos de idade, sexo masculino, procurou atendimento referindo apresentar "verruga" em borda interna de vermelhão do lábio, próximo à comissura bucal esquerda. Baseado nas características clínicas da lesão, o diagnóstico foi de papiloma escamoso oral. Optou-se pela criocirurgia por se tratar de uma técnica cirúrgica minimamente invasiva e apresentar vantagens teóricas em relação à técnica cirúrgica convencional, principalmente no que diz respeito ao manejo do paciente. Para tal, foram utilizados instrumentos simples e relativamente acessíveis, como garrafa térmica para acondicionamento do gás nitrogênio sob sua forma líquida e swab clínico para aplicação do gás sobre a lesão. Considerações finais: uma semana após o procedimento, houve a eliminação da lesão e uma boa cicatrização do local. O agente de congelamento escolhido e a técnica utilizada provaram-se eficientes como alternativa de tratamento não traumático para o papiloma escamoso.
Objective: to report the diagnosis and treatment of a possible squamous papilloma in a child using cryosurgery with liquid nitrogen. Case report: an 8-year-old male patient sought assistance claiming to present a "wart" on the inner edge of the lip vermilion, near the left oral commissure. Based on the clinical features of the lesion the diagnosis was an oral squamous papilloma. Cryosurgery was chosen for treatment because of his minimally invasive surgical characteristics and theoretical advantages relating to the conventional surgical technique, especially with regards to patient management. Hence, the instruments used for the technique were very simple and relatively inexpensive, such as a thermos bottle for conditioning of the liquid nitrogen, and a clinical swab to be applied on the lesion. Final considerations: one week after the procedure we found the elimination of the lesion and a good local healing. The freezing agent and the chosen technique have proved to be effective as an alternative of non-traumatic treatment for squamous papilloma.